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Tenfell
Awarded contract
Published 22 Aug 2024

RBGKEW1309 Long-Read Ash Genome Sequencing 2024

Royal Botanic Gardens, Kew · United Kingdom

Key facts

Contract value
£150,000
Awarded
25 Jul 2024
Contract starts
18 Aug 2024
Contract ends
18 Aug 2025
Procedure
Open procedure
Buyer type
Other public body

Awarded to

SupplierValue
NOVOGENE (UK) COMPANY LIMITED£150,000

Description

Royal Botanic Gardens, Kew wishes to appoint a supplier to individually sequence the whole genomes of up to 300 European ash trees (Fraxinus excelsior) at a minimum of 15x whole genome coverage via a long-read technology. Fraxinus excelsior is a diploid and has a 1C genome size of 880 Mbp.

In addition to the 300 individual ash trees, we wish to sequence a minimum of six technical replicates. Therefore, the total number of samples we wish to sequence is 306.

RBG Kew intends to supply total genomic DNA for each sample with the following metrics: minimum of 10ug DNA; minimum concentration of 30ng/ml (as measured with a Quantus flurometer dsDNA assay); at least 50% of the sample in fragments with a minimum length of 40kb; minimum OD260/280 of 1.8; minimum OD260/230 of 1.8; minimum DIN of 7.

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