CEFAS22-26 RFQ for DNA extraction, metabarcoding, and bioinformatics of marine environmental samples for Atlantic Bluefin Tuna (ABFT)
Defra Network eTendering Portal · United Kingdom
Key facts
- Contract value
- £20,880
- Awarded
- 19 Apr 2022
- Contract starts
- 19 Apr 2022
- Contract ends
- 30 Jun 2022
- Procedure
- Open procedure (below threshold)
- Buyer type
- Central government
Awarded to
| Supplier | Value |
|---|---|
| Applied Genomics | £20,880 |
Description
Cefas has contracted the supplier to provide services for DNA to be extracted from 116 frozen Sterivex cartridges (Millipore; SVGP01050). The cartridges consist of 29 samples in triplicate, and 29 field blanks. Following extraction, Cefas require half the extracted DNA to be sent back to the Cefas laboratory in Lowestoft so we can undertake qPCR (e.g., each of the extracted samples will be halved, one half will be used for metabarcoding, while the other half will be used for qPCR).
Cefas require the extracted DNA to undergo metabarcoding using the MiFish 12s marker (Miya et al., 2015) (and MiFish T markers if dual amplicon approach can be achieved). The supplier will also conduct taxonomic assignment on the metabarcoding data using amplicon sequence variants (preferred) or operational taxonomic units and Blast on a Genbank generated database (or similar). Metabarcoding results should be presented to Cefas so that the number of sequences per taxa is available for every sample in a table format.
The supplier will also provide the raw data for the sequencing run and methodology involved. Cefas will report results in a scientific manuscript, in consequence details on the methodology provided by the supplier will need to be sufficient to pass peer review (proprietary techniques will be protected).
The supplier will provide weekly updates via email detailing progress on deliverables.
The supplier will provide all deliverables within 10 weeks of Contract start date.
Categories (CPV)
- 71620000 Analysis services
Similar open tenders
Unclassified Road Conditions Survey (Lot 1) (2027)
Location: South EastBuyer: West Sussex County Council
Open tenderCloses 16 Nov, 202611 daysValue £75.00kSource Find a TenderWest Sussex County Council are seeking to and identify suitably qualified and interested organisations for the provision of Unclassified Road condition surveys during 2027.
Lot 4 - SCRIM Road Surveys (2027)
Location: South EastBuyer: West Sussex County Council
Open tenderCloses 16 Nov, 202611 daysValue £30.00kSource Find a TenderWest Sussex County Council are seeking to and identify suitably qualified and interested organisations for the provision of Sideway-force Coefficient Routine Investigation Machine Road Surveys during 2027.
A, B & C Road Conditions Survey (Lot 2) (2027)
Location: South EastBuyer: West Sussex County Council
Open tenderCloses 16 Nov, 202611 daysValue £90.00kSource Find a TenderWest Sussex County Council are seeking to and identify suitably qualified and interested organisations for the provision of A, B and C road condition surveys during 2027.
Lisnamult Access Road N63
Location: IrelandBuyer: Roscommon County Council
Open tenderCloses 3 Dec, 202628 daysValue €865.00KSource TEDConsisting of a new access road from the N63 to the townland of Lisnamult to access land with a potential for housing development. The project aims to provide active travel facilities
Ireland West SDZ Designated Activity Company DAC is seeking consultancy services to support the development of an Aviation Circular Economy Hub at Ireland West Airport Knock.
Location: IrelandBuyer: Mayo County Council
Open tenderCloses 24 Nov, 202619 daysValue €125.00KSource TEDMCC are publishing this competition on behalf of Ireland West SDZ Designated Activity Company. The brief is to undertake the market validation, technical, commercial and operational planning development work necessary to establish the proposed Hub’s viability. The tenderer shall identify an appropriate operating model
Provision of Site Assessment Services to the Abbey Theatre
Location: IrelandBuyer: Abbey Theatre Amharclann na Mainistreach
Open tenderCloses 9 Dec, 202634 daysValue €139.00KSource TEDThe procurement of services to carry out a Building Condition Survey, which documents the building prior to change and gives an independent opinion on its condition as well as recommendations for the level of intervention required to meet the operational, accessibility, sustainability and technical requirements of a mo