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Tenfell
Awarded contract
Published 22 May 2019

PHE_Scientific_ Analysis of iPSCs by G-banding

Public Health England · United Kingdom

Key facts

Contract value
£23,000
Awarded
21 May 2019
Contract starts
21 May 2019
Contract ends
21 May 2021
Procedure
Open procedure (below threshold)
Buyer type
Central government

Awarded to

SupplierValue
Cell Guidance System Ltd£23,000

Description

Public Health England (PHE) has awarded a contract to Cell Guidance Systems to assess the chromosomal stability of human induced pluripotent stem cells (iPSCs).

The service subject to this contract are: - Be able to receive, handle and process live cells that are Advisory Committee on Dangerous Pathogens (ACDP) Hazard Group 2. - Have experience handling and fixing human induced pluripotent stem cells (iPSCs) e.g. identifying colony cultures that are in the log phase of growth. - Use Colcemid for arresting cells in metaphase so that chromosomes can be separated for cytogenetic studies. - Fix the cells using potassium chloride (KCl) solution and 3:1 methanol: acetic acid. - Assess the karyology of the fixed cells by G-banding - at least 20 G-banded metaphase spreads needed for each sample. - Provide a summary of the representative karyotype, along with additional information if any karyotypic abnormalities are found.

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