Tender for the Supply and Installation of FRET/Ca2+ Imaging and 4-Camera TIRF Microscopes
THE UNIVERSITY OF BIRMINGHAM · West Midlands
Key facts
- Closing date
- 08 Nov 2017
- Contract starts
- 01 Dec 2017
- Contract ends
- 30 Mar 2018
- Procedure
- Open procedure (above threshold)
- Buyer type
- Education
Description
The FRET/Ca2+ imaging and 4-camera TIRF microscopes are to be purchased by the Institute of Metabolism and Systems Research (IMSR), UoB. Match funding will be provided through COMPARE (http://www.birmingham-nottingham.ac.uk/compare/index.aspx). The systems will be housed in the recently refurbished rooms of the Centre of Membrane Proteins and Receptors (COMPARE). Access will be granted to members of COMPARE, as well as the intravital microscopy (IVM) user group.
The specification below specifications outline the requirements for: a. A FRET/Ca2+ imaging microscope, which must be capable of performing fast high-sensitivity ratiometric imaging and photometric measurements in cells expressing FRET reporters or loaded with Ca2+ indictors (e.g. Fura-2). The system should be equipped with a monochromator for maximal flexibility and speed in the selection of the excitation wavelength, a dual-camera beam splitter, a very high sensitivity camera (EMCCD or equivalent). In addition, it should be equipped with a photomultiplier-based ratiometric acquisition system allowing ultrafast kinetics measurements (up to 100 microsecond temporal resolution). The system should also be equipped with a caged incubator for temperature control and a laminar-flow superfusion system (or more if needed) with compatible imaging chamber allowing both ultrarapid exchange of solutions (= 56 frames/s in normal full-frame 512x512 mode and >= 560 frames/s from a 128x128 sub-array in crop mode). The system should also be equipped with a caged incubator for temperature control and a laminar-flow superfusion system (or more if needed) with compatible imaging chambe...
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Is a Recurrent Procurement Type? : No
Categories (CPV)
- 38510000 Microscopes
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